FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_22_nlp7_no_stomata_control_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_22_nlp7_no_stomata_control_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7431004
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC130640.1758039694232435TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26800.037.48914344
CACACGT30000.036.26572812
ACGTCTG30150.035.97048615
TATGCCG28150.035.44492746
GTATGCC28700.035.00657745
GCACACG33650.032.95577211
CTCGTAT30450.032.1882242
TCGTATG31200.031.86560243
CACGTCT35200.031.40584614
AGTCACT32900.030.95388428
CTAATCG34150.030.02744133
ACACGTC37200.029.81140113
AGCACAC37450.029.61199610
CTGAACT40000.027.1138519
CGTCTGA40850.026.63405616
CAGTCAC38000.026.1550627
ATGCCGT39500.025.70189547
GAGCACA43600.025.515319
ACTAATC39700.025.12372832
TAATCGA39850.024.9428534
Produced by FastQC (version 0.11.5)