FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_21_nlp7_no_stomata_N_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_21_nlp7_no_stomata_N_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7644674
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC108420.14182422952240997TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22650.038.79448744
TATGCCG23850.036.99031446
ACGTCTG25450.036.7036715
AGTCACG24450.035.64019428
GCACACG28800.033.40460611
AGCACAC28950.033.23130810
GTATGCC26450.033.21990245
CTCGTAT26700.032.51339342
TCGTATG27600.032.34350643
GTCACGA27800.032.22324429
CACGTCT29150.031.92403414
CACACGT30250.030.99385512
ACGACAT31100.028.36048735
ATGCCGT33400.026.72784247
GAGCACA38000.026.4217539
ACCTACT23200.026.39860533
CAGTCAC34600.025.58879327
GTACTCT36950.025.1861483
TCGGAAG38050.024.8258253
ACACCTA24650.024.70086331
Produced by FastQC (version 0.11.5)