FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_20_nlp7_no_stomata_N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_20_nlp7_no_stomata_N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495078
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG18650.022.32211315
CGTATGC17000.022.03500246
TATGCCG17700.021.5626348
AGTCACG18500.020.43053228
CACACGT20750.019.55646312
CTCGTAT20000.018.90377644
GTCACGT20700.018.4261129
CACGTCT23200.018.24544714
GTATGCC21350.018.20105747
GGACTGT27850.016.973263
ACACGTC24750.016.96132713
TCGTATG22400.016.87798145
GTACTCT32150.016.4318523
GCACACG27350.016.24414411
TACTCTA33000.016.2208924
CAGTCAC24550.015.96580627
ATGCCGT24700.015.73503849
AGGACTG29800.015.628562
GTTACCT32750.015.59547714
TCTCGTA24500.015.574343
Produced by FastQC (version 0.11.5)