FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_1_wt_stomata_N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_1_wt_stomata_N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11334099
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC25150.015.577939
CGGGGGG9350.015.3421521
CCCTACA29600.014.53600310
CTACACG24650.013.76550612
CCTACAC33550.012.40760611
TACACGA29050.012.16226713
CTAGTTG32450.010.779868
GGGGTAT10150.010.6858641
CCGGGAT30450.010.4560611
TTCCCTA42250.010.4320978
GCCGGAT33550.010.4284921
GCACACG21900.010.2227911
GGGGAAT39400.010.2121141
ACACGAC34100.09.95081314
GCGGAAT24150.09.8515791
CGCGGGG12300.09.6713671
GCGGATC37650.09.5705822
TATGCCG19500.09.5168848
CGCGGAT31300.09.5014231
GCCGGGT29350.09.4174441
Produced by FastQC (version 0.11.5)