FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_19_nlp7_no_stomata_N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_19_nlp7_no_stomata_N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10709225
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC138160.12901026918381114TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30300.032.58346646
CGTATGC30800.031.71291444
ACCTATA31800.030.81538232
GCACACG37250.029.58246211
ACGTCTG36600.029.4397315
GTATGCC34200.028.7639645
CACCTAT34650.028.37939631
CACACGT38950.028.20149812
CCTATAC36000.027.31823333
AGCACAC41600.026.4050110
CACGTCT41800.026.02784314
CTCGTAT38200.025.65918542
TCGTATG38750.025.47733543
TCACCTA39650.025.24242430
CTATACA41250.024.0118434
ACACGTC45900.023.4742713
ATGCCGT45000.021.93871347
CGTCTGA50650.021.75691816
GTCACCT45800.021.39353229
TGCCGTC49700.020.07759948
Produced by FastQC (version 0.11.5)