FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_18_wt_no_stomata_control_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_18_wt_no_stomata_control_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10913344
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284620.26079998944411537No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT198250.18165834413356713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC176530.16175610335383914TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG33550.036.81727631
CGTATGC39350.032.29434244
ACCTAGC38700.032.10277632
ACGTCTG44450.031.08775115
GTATGCC41150.030.71029745
CACGTCT46500.030.24315614
TCGTATG42150.029.73295243
CTCGTAT41850.029.35813342
TCACCTA43950.029.0606630
CACACGT48550.029.03781112
TAGCTAT43500.028.48399535
TATGCCG45500.027.77718546
GCACACG54100.027.02894211
GTACTCT89750.026.9356523
AGCACAC54300.026.8005410
CCTAGCT47800.026.21128533
TATCTCG46850.026.07394439
TACTCTA92700.025.9273954
ACCTACT57650.025.61806133
GTCACCT48900.025.61767229
Produced by FastQC (version 0.11.5)