FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_17_wt_no_stomata_control_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_17_wt_no_stomata_control_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20644365
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC521040.2523884847027264TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC86250.043.5516444
ACGTCTG98500.042.2655315
TATGCCG90000.041.50551646
CTCGTAT94250.040.03746442
GCACACG104750.039.8762511
CACACGT104550.039.8186912
GTATGCC95450.039.46210545
TCGTATG96500.039.1791643
AGCACAC111350.037.76393510
CGGAATA102350.036.75889634
CACGTCT117200.035.78975714
TATCTCG105350.035.5179739
ACACGTC120900.034.37615613
CCAGTCA122550.030.8690826
CAGTCAC123750.030.65487927
ATGCCGT123800.030.25818347
AGTCACC126700.030.02442728
GAGCACA142100.029.9858389
CTGAACT140100.029.74157319
GCCGTCT127000.029.7194749
Produced by FastQC (version 0.11.5)