FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_16_wt_no_stomata_control_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_16_wt_no_stomata_control_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12135880
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG37950.015.6710648
CACCTAC28800.015.43285232
ACCTACT30650.015.07257233
GGACTGT39050.014.97450363
TACTCTA40750.014.9389054
CCTACTA28350.014.81460134
ACACCTA29800.014.79521431
CCTACAC35550.014.07212211
TCCCTAC29000.013.9933979
AGGACTG41550.013.90547962
CTGTAGG30650.013.822391549
ACTCTAG46900.013.4276065
CGTATGC21500.013.35205744
GTACTCT46800.013.3067833
GTAGGAC30800.013.30019851
TCTAGTT51350.013.2848157
CTACACG27400.013.15067512
GACACCT43700.012.81155830
ACGTCTG24050.012.80092915
CCCTACA39600.012.456243510
Produced by FastQC (version 0.11.5)