FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_15_wt_no_stomata_N_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_15_wt_no_stomata_N_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7161064
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16400.021.98800544
TATGCCG16750.021.53005846
ACGTCTG17900.021.10670315
CACACGT18200.020.75734112
GCACACG18450.020.47621711
GTATGCC18450.019.73409745
AGCACAC20200.018.87545610
ACACGTC21350.017.53118513
CTCGTAT20850.017.46169342
TCGTATG21500.016.77189443
CGGGGGG7600.016.1133561
ATGCCGT23350.015.89331347
CTAGTTG22550.015.66738
GGACTGT20150.015.63963763
CACGTCT24300.015.25917414
CTACACG16300.014.80746712
TATCTCG24750.014.42575439
CCCTACA21350.014.09038710
TCCCTAC18700.014.0294949
CCTACAC21500.013.99208311
Produced by FastQC (version 0.11.5)