FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_14_wt_no_stomata_N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_14_wt_no_stomata_N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8222281
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5100.014.4077331
GGACTGT24800.013.97832463
CCTACAC27300.013.58263711
CTACACG15400.013.40216212
CCCTACA25700.013.33924410
TCCCTAC18850.013.1760519
TAGCCCG14050.012.9529534
CGGGGGG8450.012.8366461
TACTCTA27650.012.7791364
CTAGTTG29250.012.6769738
CCTACTA17100.012.48462234
TCTAGTT31750.012.339857
AGGACTG27600.012.17999162
TAGTTGT29950.012.147169
GTACTCT31300.011.735933
CGGCAAT26400.011.66340351
TACACGA19250.011.2669713
CTAATGC31550.011.08864521
ACCTACT21250.011.03408633
CACCTAC19750.010.985805532
Produced by FastQC (version 0.11.5)