FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_13_wt_no_stomata_N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_13_wt_no_stomata_N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11754068
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25600.025.02614844
TATGCCG25650.024.56946846
ACGTCTG31100.023.17165615
GTATGCC28100.022.9238245
GCACACG31350.022.7623411
CACACGT31700.022.06981812
CAGGCGA31350.020.76352734
AGCACAC36100.020.54248410
CCAGGCG34000.019.04169533
ACACGTC39100.018.07200413
CTCGTAT34650.017.98240942
CACGTCT39300.017.98018814
ATGCCGT35300.017.95153247
TCGTATG37300.017.45695543
TGCCGTC38650.016.85064748
AGGCGAT39750.016.28917735
CGTCTGA46650.015.67293616
CGGGGGG9200.015.5929221
ACCAGGC43300.015.19430832
TCCCTAC29100.015.1461369
Produced by FastQC (version 0.11.5)