FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_12_nlp7_stomata_control_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_12_nlp7_stomata_control_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15537256
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT203540.13100125273085544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG10750.022.78481
CGCGGAT86100.017.4345111
TAGGGTA64900.016.55275331
CGGGCAT15600.016.1496661
GCGGATC102700.015.2622462
GGGGTAT13300.015.2592081
CGAGTTA72250.014.96214316
GGGGGGC7250.014.9601042
GCCGGGT37700.014.9430821
CGCGGGG7200.014.5795591
GATAGGG74650.014.48437229
GGATCTA112200.014.4371314
CCCTACA28950.014.38004910
GCTATAC75900.014.11760961
ACAGTGC103550.013.96436868
CAGTGCC103300.013.96359469
TCCCTAC27200.013.8904689
AGTGCCT109300.013.35722570
CGGGGGT11850.013.28771
CGTAATA117200.013.07433715
Produced by FastQC (version 0.11.5)