FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_11_nlp7_stomata_control_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_11_nlp7_stomata_control_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11739987
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT13850.014.6540771
CCCTACA24850.014.35913510
TAGGGTA39100.013.96107431
GTCCGCT9600.013.8513661
CGGGGGG8850.013.8390091
TCCCTAC22000.013.8341049
CTACACG22300.013.49106412
GCTATAC40950.013.2543961
ACAGTGC54550.013.157866568
TACACGA24100.013.06416813
GCGGGGG11650.012.9158441
CCTACAC30650.012.89735211
CAGTGCC57250.012.47526169
GGGGTAT10400.012.4494071
CACAGTG59900.011.98317667
AGTGCCT60850.011.85222170
CGCGGAT66600.011.8219351
CCGGGAT22600.011.7675321
GGGGAAT41500.011.6362021
TATACAC46100.011.62098263
Produced by FastQC (version 0.11.5)