FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_10_nlp7_stomata_control_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_10_nlp7_stomata_control_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13116247
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT164700.12556945595794286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG7850.018.2760661
GCACACG19100.017.94934511
CTACACG18950.017.16839212
ACGTCTG22050.015.23140815
GGGGTAT11050.015.2001361
TCCCTAC25050.014.9427999
CCCTACA30150.014.50369310
CCTACAC32600.014.05754811
CGCGGAT73200.014.0063511
CGCGGGG8550.013.9149381
TAGGGTA50700.013.52747131
CGGGCAT14300.013.4584531
CGTATGC17900.013.29953244
TATGCCG17350.013.11673846
GCCGGGT30100.013.020271
CACACGT25850.012.85638812
CGTAATA91100.012.78801915
GGGGCAT16250.012.7047811
GCGGATC88400.012.3881822
TACACGA26700.012.31626713
Produced by FastQC (version 0.11.5)