FastQCFastQC Report
Thu 21 Nov 2019
HJ5THBGXC_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5THBGXC_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470172158
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG30044820.6390174213590929RNA PCR Primer, Index 41 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGT11687300.044.0123251
TCCACGA248086500.043.85877
AACGTCC12917000.043.846813
GGTCTCT248950450.043.8410414
AGGTCTC250092450.043.83423613
CCACGAG249797750.043.8236928
GAGGTCT249957850.043.82096512
ACGAGGT250008200.043.8191210
CACGAGG250062850.043.796079
GTCTCTT50126950.043.78582815
GTCTCTA75440300.043.7700115
GGTCCAC100770450.043.7515035
ACTGTCC16064450.043.720633
AATGGTC15660600.043.718792
ACGTCCA13531800.043.7173924
CGTCCAC64515050.043.6953475
AACTGTC16055800.043.6911132
GTCTCTG65145000.043.6903815
GGGTCCA11881550.043.6716354
TGTGTCG687900.043.63030236
Produced by FastQC (version 0.11.5)