HJ5TCBCXX_l02n02_t4ydrt.352000000589dc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HJ5TCBCXX_l02n02_t4ydrt.352000000589dc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1395566
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	61324	4.394202782240324	Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	4606	0.3300453006163807	Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	2532	0.18143176316992532	Illumina Single End PCR Primer 1 (100% over 47bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	1885	0.13507064517192308	Illumina Single End PCR Primer 1 (100% over 41bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1799	0.12890827090943746	Illumina Single End PCR Primer 1 (98% over 51bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	4545	41.88009	18
AGAGCAC	6745	41.596386	15
AGCACAC	4920	41.43184	17
GAGCACA	6720	41.416325	16
CACACGT	3320	41.066727	19
ACACGTC	2055	39.523094	20
CACGTAG	1330	39.245686	21
AGATAGA	5040	39.24426	7
CACACGA	1360	39.041695	19
AAGAGCA	8935	38.80424	14
ACACGAG	1375	38.452156	20
ACACGTA	1400	38.40833	20
GTCGCCG	8880	38.131077	45
CACGTCT	1080	37.91431	21
AGCACAA	1880	37.818153	17
GCACAAG	1900	37.420067	18
TAGATAG	1725	37.30738	6
CACGAGA	1430	37.13056	21
GATAGAT	5340	37.12247	8
CAGATAG	1685	36.99114	6