FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t4yd37.3520000005897f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t4yd37.3520000005897f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13889723
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7132195.134868420342148Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT537300.3868327683712627Illumina Single End PCR Primer 1 (100% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT391340.2817478793493578Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG272890.19646900085768448Illumina Single End PCR Primer 1 (100% over 41bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG236910.17056495655096937Illumina Single End PCR Primer 1 (98% over 51bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT233030.16777152431333583Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT222320.16006078738935253Illumina Single End PCR Primer 1 (100% over 39bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC192850.13884366160505865Illumina Single End PCR Primer 1 (100% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG164590.11849768350311954Illumina Single End PCR Primer 1 (98% over 51bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC162010.11664019505644568Illumina Single End PCR Primer 1 (100% over 50bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG158860.11437233125527414Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG153540.11054216127996216Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG650850.041.39306618
CACACGT486850.041.34249519
ACACGTC304300.041.26416420
AGCACAC689350.041.15054717
GAGCACA963950.040.94666716
AGAGCAC972900.040.82915
AGATAGA813600.040.06957
CAGATAG282200.039.8340956
AAGAGCA1296750.039.47941214
CACGTCT155550.039.34192321
CACGTAG185600.039.06960721
ACACGTA187650.038.9910520
CACGTCA156800.038.942221
CACACGA172100.038.84049619
ACGTCAG155600.038.77968622
ACACGAG171900.038.38832520
GATCGAG127350.037.986078
GATAGAT863650.037.799378
GCACAAG280700.037.70458218
GTCGCCG1102350.037.5504945
Produced by FastQC (version 0.11.2)