FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t4mr.3520000005892f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t4mr.3520000005892f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15570162
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG392320.25196911888264234Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG217850.040.3098518
CACACGT171850.039.01447319
AGCACAC235650.038.63989617
AGAGCAC268600.037.903815
GAGCACA268850.037.6928116
ACACGTC125950.037.1555920
ACGTCAG75750.033.77001622
CACACGA62450.033.43293819
GTCGCCG69300.033.1477745
GGTCGCC68300.033.1059344
CACGTAG62450.032.7481121
ACACGAG65000.032.12133820
CACGTCT67050.031.57516721
AAGAGCA350900.031.29648814
ACACGTA66000.031.02104820
CACGAGA70100.029.6878421
CGTCTGA55500.029.63315223
CGTCAGA87600.029.53569423
ACGTCTG56350.029.06637622
CACGTCA90950.028.17577621
Produced by FastQC (version 0.11.2)