FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t3ydrt.352000000589b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t3ydrt.352000000589b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11997352
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3058092.5489708062245735Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT257460.21459735448288922Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT123860.10323944817156319Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG184600.039.87994818
CACACGT141900.039.38503619
GAGCACA262800.038.87756316
AGAGCAC265550.038.7969215
AGCACAC206400.038.49115817
ACACGTC99000.038.11184720
GTCGCCG460650.037.76489645
AGATAGA245350.036.0323377
GGTCGCC486300.035.69880344
AAGAGCA364300.035.3087214
CACGTCT58450.035.10410321
GTGGTCG501850.034.5831242
GATAGAT256100.034.4052058
TGGTCGC506100.034.22659743
CACACGA51550.034.13030219
ACACGAG51900.033.16317720
GGTGGTC523900.033.1314641
CGGTGGT527350.032.91030540
CAGATAG96450.032.870836
ACACGTA52350.032.79215220
Produced by FastQC (version 0.11.2)