FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t3yd37.35200000058962.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t3yd37.35200000058962.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14941147
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2862791.916044330465392Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT259440.17364128737907472Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT240570.1610117349089732Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG323750.040.54426618
AGCACAC344050.039.7215417
CACACGT240850.039.5423819
GAGCACA475250.039.45046216
AGAGCAC481300.039.17427415
ACACGTC160550.038.8317920
AAGAGCA670800.036.65727214
CACGTCT96250.036.32500521
ACACGAG97250.036.09077520
CACACGA98600.035.93890419
CACGTAG84550.035.84341421
GTCGCCG483750.035.72871845
AGATAGA316500.035.3463677
ACGTCTG76450.034.57926622
ACACGTA89450.034.458920
CGTCTGA70050.034.3340423
GCACAAG142800.033.9698318
CACGAGA103750.033.80752621
GGTCGCC512750.033.6465544
ACGTCAG75700.033.52498622
Produced by FastQC (version 0.11.2)