FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t3mr.35200000058905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t3mr.35200000058905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13753
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG290.21086308441794518Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG240.17450738020795462Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATAGATCGGAAGAGCGTCGTG170.12360939431396785Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG140.10179597178797353Illumina Single End PCR Primer 1 (100% over 28bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC900.045.00000420
CACACGT900.045.00000419
GAGCACA900.045.00000416
AGCACAC900.045.00000417
GCACACG950.045.00000418
ACGTCTG750.045.022
TGACTGG206.792666E-445.02
TGGAGTT253.692815E-545.06
GTTCAGA253.692815E-545.010
TGGAGAG206.792666E-445.06
TGCTCTT253.692815E-545.021
CTGGAGT206.792666E-445.05
GTGACTG206.792666E-445.01
TCTTCCG206.792666E-445.024
TTCAGAC253.692815E-545.011
GTGTGCT253.692815E-545.018
AGTTCAG253.692815E-545.09
TGTGCTC206.792666E-445.019
GGAGTTC253.692815E-545.07
ACGTGTG253.692815E-545.016
Produced by FastQC (version 0.11.2)