FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t3d37.35200000058a3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t3d37.35200000058a3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16628062
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2409291.448930127876598Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT187780.11292957651950059Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG275400.040.10543418
CACACGT214650.039.40033319
GAGCACA369200.039.0268816
AGCACAC295650.038.8424817
AGAGCAC374250.038.8188915
ACACGTC150850.038.0471720
GTCGCCG378400.037.33342745
GGTCGCC392100.035.9657744
CACGTCT90350.035.30997521
TGGTCGC403850.034.76892543
AAGAGCA501150.034.6775714
GTGGTCG410700.034.2271642
AGATAGA255650.033.630067
CACGTAG74950.033.0795321
CACACGA76600.033.07258219
ACACGAG74750.033.01814320
GGTGGTC429500.032.77592541
ACGTCTG69700.032.66608422
ACGTCAG71400.032.32946422
CTCGGTG432750.032.30111738
Produced by FastQC (version 0.11.2)