FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t2ydrt.35200000058999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t2ydrt.35200000058999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14229263
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1026430.7213514853158594Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG85650.036.9608818
GTCGCCG162400.036.3116445
GGTCGCC165900.035.3692744
CACACGT74700.034.51679219
AGCACAC100600.033.48107517
GAGCACA121700.033.3519716
AGAGCAC124500.033.12597715
ACACGTC59200.032.98852520
GTGGTCG178500.032.60734642
TGGTCGC183250.031.67675243
CTCGGTG185050.031.22195638
GGGGGAT54400.030.7063271
TCGGTGG199350.029.10645339
GGGGGTA66700.029.0265561
GGGGGTC40650.028.9089871
GGGGGAC43400.028.4258671
CGGTGGT204950.028.38840
TCTCGGT204450.028.36938937
CACGTCT45100.027.9854921
GGTGGTC213650.027.26360941
Produced by FastQC (version 0.11.2)