FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t2yd37.35200000058949.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t2yd37.35200000058949.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15048626
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3563142.367751049165552Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT289040.19207069137076035Illumina Single End PCR Primer 1 (100% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT282460.18769819915785002Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG294200.040.35656718
CACACGT219800.039.78775819
AGCACAC318150.039.150217
ACACGTC151200.039.0458320
AGAGCAC435650.038.75475315
GAGCACA434250.038.70871416
AGATAGA398850.036.7643367
GTCGCCG597050.036.3646345
AAGAGCA609400.035.92732214
CACGTCT95400.035.8700421
CACACGA86450.035.36853419
GATAGAT418000.035.0095948
CAGATAG145250.034.7460986
ACACGAG87100.034.6654620
CACGTAG76200.034.51521721
GGTCGCC636050.034.09599344
ACGTCTG76050.033.93245322
ACACGTA79450.033.86880520
TGGTCGC647100.033.32253343
GTGGTCG650900.033.16590542
Produced by FastQC (version 0.11.2)