FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t2mr.352000000588dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t2mr.352000000588dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14456050
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG735730.5089426226389643Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG346050.040.81166518
AGCACAC366650.040.04667317
CACACGT278800.040.01873819
AGAGCAC417350.039.7641315
GAGCACA420350.039.4000516
ACACGTC195650.038.88009620
ACGTCAG125300.037.50903322
CACGTAG96350.036.9405421
CACACGA82450.035.9109819
AAGAGCA505850.035.44484714
GTCGCCG125700.035.44009845
ACACGTA101550.035.09435720
CACGTCA134800.034.8322121
ACACGAG84250.034.8232820
CGTCAGA136900.034.64301323
GGTCGCC126600.034.6193444
CACGTCT85350.034.26796321
CGTCTGA68400.032.39877723
CACGAGA92300.031.85828421
TGGTCGC134950.031.47694843
Produced by FastQC (version 0.11.2)