FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t1d37.35200000058a2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t1d37.35200000058a2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12225935
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1037110.8482868590418647Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG143100.040.0779518
CACACGT117050.038.53994819
AGCACAC157100.037.78103617
GAGCACA196100.037.6217616
AGAGCAC198050.037.5580715
ACACGTC84850.036.99053220
GTCGCCG167200.036.96483645
GGTCGCC171350.035.97765744
GTGGTCG181450.033.76397342
CACGTCT59200.033.59576421
TGGTCGC182350.033.47421643
CTCGGTG186450.032.43565438
AAGAGCA278350.032.22777614
CACGTAG40250.031.6935721
GGTGGTC196700.031.19177441
CACACGA38300.030.78227219
ACGTCTG50500.030.74042922
TCTCGGT197650.030.65457537
TCGGTGG198300.030.62216839
CGGTGGT202050.030.09836840
Produced by FastQC (version 0.11.2)