FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n02_t0d37.35200000058a04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n02_t0d37.35200000058a04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18232249
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1442150.7909885390441959Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG162600.038.82768218
CACACGT134550.036.80411519
GTCGCCG227000.036.66270445
AGCACAC179700.036.5602517
GAGCACA226800.036.30884616
AGAGCAC228100.036.26959615
GGTCGCC235500.035.3298744
ACACGTC100300.033.82667520
TGGTCGC249850.033.2016643
GTGGTCG252500.032.87030842
CTCGGTG257450.031.83605238
AAGAGCA330250.030.89646714
GGTGGTC272850.030.4020841
TCTCGGT271900.030.1359537
CACGTCT71050.029.9555721
TCGGTGG277150.029.76795239
ACACGAG43350.029.06416120
CGGTGGT286450.028.89574440
CACACGA47450.027.45379619
CACGTAG46800.027.1614621
Produced by FastQC (version 0.11.2)