FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t4ydrt.351000000589df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t4ydrt.351000000589df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395566
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC702285.032223484951626TruSeq Adapter, Index 16 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC48830.34989387818275886TruSeq Adapter, Index 16 (98% over 51bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA18730.13421077899576228TruSeq Adapter, Index 16 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC91350.038.6961545
TCGTATG95500.036.9439144
CTCGTAT94250.036.8847943
CATCTCG89600.036.58909640
TCTCGTA91150.036.06564742
ATCTCGT92300.035.5919141
CCATCTC96800.033.79786339
GTCCATC98000.033.26682337
TCCATCT98800.033.06577738
CACCTCG2800.030.53695340
CGTCCAT110900.029.66093836
GTATGCC12550.027.78997245
ACCTCGT3250.027.69343241
GTCCACC3450.024.7818437
CGTCCAC4350.024.30959136
CCTCGTA3450.024.13141442
ACTCGTA3450.023.47921442
CAACTCG3850.021.62425440
CCGTCCA165350.021.17261135
AACTCGT4150.021.1454441
Produced by FastQC (version 0.11.2)