FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t4yd37.35100000058972.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t4yd37.35100000058972.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13889723
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC8583036.1794104893236526TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC429550.30925742723594996TruSeq Adapter, Index 9 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC281880.2029414121505519TruSeq Adapter, Index 9 (98% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA273700.1970521658351286TruSeq Adapter, Index 9 (100% over 40bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG234420.16877226421290042TruSeq Adapter, Index 9 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA225700.16249424124584774TruSeq Adapter, Index 9 (100% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT201970.145409667277022TruSeq Adapter, Index 9 (100% over 51bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG170560.12279582537391134TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC152630.1098870006262904TruSeq Adapter, Index 9 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1123100.038.96484845
TCGTATG1197200.036.52495244
CTCGTAT1191950.036.1685943
TCTCGTA1127050.035.97141642
ATCTCGT1146300.035.337941
AGATCTC1181950.034.0930939
GATCTCG1202150.033.62690440
CAGATCT1207150.033.3256438
GCCGTCT47600.029.73293744
TCAGATC1468000.027.3822337
GACCTCG27850.026.58060340
CCGTCTT55100.026.4208545
ATCAGAT1597850.025.43344536
ACCTCGT29500.025.32270841
CCTCGTA30600.024.41241642
TGCCGTC59150.024.11729843
ACTCGTA57700.024.09943242
GAACTCG59000.023.75910840
GAGCTCG20500.023.48843440
ATGCCGT62650.022.9854542
Produced by FastQC (version 0.11.2)