FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t4mr.35100000058922.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t4mr.35100000058922.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15570162
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC419250.2692650211346549TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT37650.025.03901910
AGAGCGG39450.024.1246839
CTCGTAT88300.023.672243
CGTATGC90800.023.2434545
GCGGTTC43450.021.80020312
TCGTATG98550.021.39274844
TCTCGTA102550.019.92203942
ATCTCGT103050.019.38869541
GATCTCG104500.018.90435440
GACGTGT148350.018.7652191
AGCGGTT51200.018.67614411
ATGCCGA30150.018.5814826
TGCCGAG28850.018.40494227
CCGAGAG20550.017.9557329
AAGAGCG54100.017.7997868
CGGTTCA54000.017.66648513
GGAATGC46400.017.11689423
AATGCCG36200.017.09197825
CAGCAGG56850.016.38505618
AGCAGGA58450.016.01352119
Produced by FastQC (version 0.11.2)