FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t3ydrt.351000000589b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t3ydrt.351000000589b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11997352
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3550852.9596947726464973TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC279170.23269301425847969TruSeq Adapter, Index 12 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC472550.037.4820745
TCGTATG507400.034.88106544
CTCGTAT504800.034.60608343
TCTCGTA496600.033.84996842
ATCTCGT499600.033.63319841
AATCTCG498700.033.6126840
TAATCTC524800.031.9495939
GTAATCT526450.031.64311638
TGTAATC548050.030.36301437
GTATGCC77450.024.72261245
TTGTAAT679450.024.6964736
CTTGTAA929950.018.78675735
CCTTGTA975200.017.93810834
ACCTTGT1051700.016.65469633
CACCTTG1068950.016.39645832
GTCACCT1091400.016.14782730
TCACCTT1105650.015.94784931
AGTCACC1114150.015.9029229
GACGTGT117550.015.2389961
CAGTCAC1182050.015.1720828
Produced by FastQC (version 0.11.2)