FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t3yd37.35100000058965.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t3yd37.35100000058965.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14941147
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3504262.3453754922563843TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC292150.19553385024590145TruSeq Adapter, Index 8 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC490750.036.77506645
TCGTATG529700.034.0496844
CTCGTAT523750.033.9639443
TCTCGTA507150.033.39861742
AATCTCG504200.033.38875240
ATCTCGT513950.032.8998141
GAATCTC539750.030.889539
TGAATCT563450.029.63714238
TTGAATC583750.028.62182837
CTTGAAT704100.023.91834636
GTATGCC88700.022.06891345
GTGACTG40950.020.9926591
GCCGTCT31500.019.42869644
GACTGGA47550.018.5508163
TGACTGG47550.018.1722282
ACTTGAA1010450.017.54856735
CACTTGA1036650.017.08768534
TGCCGTC38400.016.3477643
GGAGTTC52850.016.3050867
ACACTTG1103600.016.05921433
Produced by FastQC (version 0.11.2)