FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t3mr.35100000058908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t3mr.35100000058908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13753
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC2762.006834872391478TruSeq Adapter, Index 14 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA300.21813422525994328TruSeq Adapter, Index 14 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG250.18177852104995273TruSeq Adapter, Index 14 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT170.12360939431396785TruSeq Adapter, Index 14 (97% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGG150.10906711262997164TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATC1056.548362E-1125.71428533
CACAGAT1056.548362E-1125.71428532
TCACAGA1055.082984E-821.42857231
GTCACAG954.7606954E-721.3157930
CAGTCAC907.3230276E-620.00000228
CAGATCG1402.384695E-919.28571334
AGTCACA1001.8186365E-518.029
GAACTCC1558.44193E-917.41935322
CTGAACT1601.2507371E-816.87520
TGAACTC1601.2507371E-816.87521
CTCCAGT1104.125933E-516.36363625
TCCAGTC1104.125933E-516.36363626
AACTCCA1551.6681224E-715.96774223
TCTGAAC1702.6446287E-815.88235319
CCAGTCA1156.0379753E-515.65217327
ACGTCTG1753.780042E-815.42857116
CACGTCT1753.780042E-815.42857115
GTCTGAA1753.780042E-815.42857118
CGTCTGA1753.780042E-815.42857117
ACACGTC1805.345828E-815.00000114
Produced by FastQC (version 0.11.2)