FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t3d37.35100000058a3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t3d37.35100000058a3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16628062
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2763961.6622261812591268TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC208350.12530023041771193TruSeq Adapter, Index 10 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC390350.036.2386145
TATCTCG394550.034.25609240
CTCGTAT411450.034.1942943
TCGTATG415250.034.11436544
TCTCGTA411200.033.10430542
ATCTCGT411000.033.0547241
GCTTATC443050.030.28675737
TTATCTC449450.030.06172239
CTTATCT453550.029.74451638
AGCTTAT541700.024.90008436
GTATGCC73200.020.5635145
TAGCTTA765950.018.19471235
CTAGCTT839800.016.61078634
GTGACTG39850.016.321111
ACTAGCT905900.015.45837133
CACTAGC921500.015.26748532
GACGTGT167750.015.1196431
TCACTAG945950.014.88475831
GTCACTA976750.014.46376830
AGTCACT984050.014.36781629
Produced by FastQC (version 0.11.2)