FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t2ydrt.3510000005899c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t2ydrt.3510000005899c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14229263
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1161320.8161490865689952TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC170600.034.185245
CATCTCG167850.032.93562340
CTCGTAT177400.032.519743
TCGTATG183650.031.73152544
ATCTCGT178950.031.04355841
TCTCGTA181950.030.49461242
ACATCTC189350.029.25531839
TACATCT210300.026.41516138
CTACATC217250.025.64243337
GCTACAT229600.024.16532336
GGCTACA304200.018.91963435
CGGCTAC321050.017.9196534
CACGGCT345250.016.68965532
ACGGCTA346750.016.62394533
TCACGGC352750.016.34756331
GTCACGG359700.016.06297530
AGTCACG367400.015.79981429
CAGTCAC405550.014.757256528
CCAGTCA433100.013.85489427
TCCAGTC437600.013.76897526
Produced by FastQC (version 0.11.2)