FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t2yd37.3510000005894c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t2yd37.3510000005894c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15048626
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4417312.9353576864758284TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC312560.20770002523818454TruSeq Adapter, Index 7 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC610300.037.02984245
CATCTCG621300.034.25928540
TCGTATG660900.034.1675244
CTCGTAT657900.034.0770843
TCTCGTA637250.033.63483442
ATCTCGT641250.033.40748241
TCATCTC699200.030.34903539
GATCATC716200.029.53607737
ATCATCT745200.028.47138838
AGATCAT848800.025.07857936
GCCGTCT40450.021.69374844
GTATGCC107700.020.36936245
TGCCGTC49300.018.16455343
CAGATCA1225350.018.12665635
CCGTCTT51150.017.41959645
GTGACTG38850.017.0302641
ATGCCGT53250.016.90164442
CACCTCG17800.016.8120340
TATGCCG55050.016.43074641
GACTGGA41150.016.3508533
Produced by FastQC (version 0.11.2)