FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t2mr.351000000588d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t2mr.351000000588d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14456050
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC812020.5617163748050125TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC144150.029.11073945
CTCGTAT143200.028.80106443
TCGTATG155300.027.0206944
GAGCGGT53850.025.5282210
TCTCGTA153500.025.2707442
AGAGCGG57050.024.3329379
ATCTCGT161250.023.93059341
GACGTGT219850.023.1552721
GCGGTTC60450.022.92711412
AGCGGTT63900.021.68926611
GATCTCG178450.021.51055140
CGGTTCA66450.020.75573213
CAGCAGG67400.020.56332418
TGCCGAG35200.020.19812227
GGAATGC56750.020.17987423
AATGCCG45850.019.97200425
CCGAGAG22800.019.73618329
ATGCCGA39700.019.6088526
AAGAGCG74100.019.007338
AGCAGGA72200.018.97809619
Produced by FastQC (version 0.11.2)