FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t1d37.35100000058a21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t1d37.35100000058a21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12225935
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1145710.937114421105625TruSeq Adapter, Index 22 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC170900.034.58608645
CTCGTAT178950.032.4267243
TCGTATG183700.032.2496644
ATCTCGT175050.031.18258541
TCTCGTA177600.030.82354542
GATCTCG181350.030.00006340
ACGATCT181900.029.83416238
TACGATC183900.029.4852337
GTACGAT224950.024.34476536
CGATCTC238500.022.792539
GTGACTG40000.021.2100261
TGACTGG45550.018.8221722
CGTACGA311200.018.7254835
GACTGGA46950.018.4526313
GGAGTTC47900.018.0839987
CCGTACG332450.017.5556334
ACCGTAC373450.015.72464433
CTGGAGT56650.015.5313795
CACCGTA382650.015.328938532
GTCACCG390350.015.03232630
Produced by FastQC (version 0.11.2)