FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l02n01_t0d37.35100000058a07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l02n01_t0d37.35100000058a07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18232249
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC1587980.8709731860287778TruSeq Adapter, Index 21 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC244350.034.03334845
CTCGTAT251550.032.28999343
TCGTATG261550.031.78664844
ATCTCGT247600.031.16940541
GATCTCG246500.031.15332440
TCTCGTA254700.030.33586542
TCGATCT253450.030.13801438
TTCGATC270450.028.21863737
CGATCTC299500.025.46008139
TTTCGAT358750.021.81264736
GTTTCGA454000.018.03430235
CGTTTCG462350.017.6842734
GTATGCC53200.016.02922845
ACGTTTC530800.015.4843133
GTCACGT531650.015.45955530
AGTCACG537600.015.35110429
CACGTTT537250.015.33610332
TCACGTT542250.015.165647531
CAGTCAC595300.014.23349728
CCAGTCA644500.013.17842727
Produced by FastQC (version 0.11.2)