FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t3rr1r201.352000000589cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t3rr1r201.352000000589cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9073255
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1926862.1236700610751047Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT115010.12675715605920918Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT97040.10695169484380193Illumina Single End PCR Primer 1 (100% over 47bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC91920.10130873650084783Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG153900.039.17939418
CACACGT117750.038.8643619
ACACGTC81550.038.37644220
AGCACAC165600.038.1503317
AGATAGA228150.037.8283357
GTCGCCG310350.037.5672645
GAGCACA218200.037.490916
AGAGCAC223200.037.02319715
CACGTCT52400.036.2387121
GATAGAT238350.036.1520658
CAGATAG88950.035.9936
GGTCGCC333800.034.79329744
ACGTCTG42550.034.1581622
CACACGA42450.034.07962419
ACACGAG41850.033.8693320
CGTCTGA40250.033.8182423
GTGGTCG341700.033.7317242
GATCGAG41350.033.6250158
ATAGATC257100.033.3755269
AAGAGCA310650.033.31415614
Produced by FastQC (version 0.11.2)