FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t3r1r201.3520000005893c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t3r1r201.3520000005893c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849002
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2405711.8722932722712626Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT162970.12683475339174202Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG225600.039.88147418
CACACGT181650.039.18861819
AGCACAC242450.038.70587517
ACACGTC128000.038.5643120
GAGCACA312200.038.20150416
AGAGCAC316550.037.8540815
GTCGCCG386350.037.10099845
CACGTCT80850.036.3152821
AGATAGA247800.035.800117
GGTCGCC409600.034.94011744
ACGTCTG65400.034.60807422
CGTCTGA61650.034.55989523
GATAGAT259100.034.1421978
AAGAGCA436500.034.11550514
CACGTAG61650.034.0857421
GTGGTCG418250.033.97530742
ACACGAG54000.033.3315220
CAGATAG97200.033.0770346
TGGTCGC430300.033.0187743
GGTGGTC434200.032.7583441
Produced by FastQC (version 0.11.2)