HJ5TCBCXX_l01n02_t2rr1r201p.35200000058a11.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HJ5TCBCXX_l01n02_t2rr1r201p.35200000058a11.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16785085
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	2510330	14.955718127134892	Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	168198	1.0020682051952672	Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	114216	0.6804612547389542	Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	91518	0.5452340574980704	Illumina Single End PCR Primer 1 (100% over 39bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	81881	0.48781999018771727	Illumina Single End PCR Primer 1 (100% over 47bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT	81219	0.4838760125432788	Illumina Single End PCR Primer 1 (100% over 51bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	77789	0.4634412039021548	Illumina Single End PCR Primer 1 (97% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	57311	0.34144003441150284	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	56352	0.335726628730209	Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG	56341	0.33566109435847363	Illumina Single End PCR Primer 1 (100% over 42bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	50471	0.30068957053241013	Illumina Single End PCR Primer 1 (100% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	46359	0.27619163084369247	Illumina Single End PCR Primer 1 (98% over 51bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	44280	0.2638056345857051	Illumina Single End PCR Primer 1 (100% over 50bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	37448	0.22310283206787457	Illumina Single End PCR Primer 1 (98% over 51bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	36054	0.2147978398679542	Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	34527	0.2057004775370515	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGTCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG	31398	0.18705892761341394	Illumina Single End PCR Primer 1 (100% over 32bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	29846	0.17781262352856717	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	27679	0.16490235229669672	Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	25521	0.15204570009624616	Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	20801	0.12392549695160913	Illumina Single End PCR Primer 1 (100% over 43bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	20613	0.12280545496194985	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG	20510	0.12219181493570036	Illumina Single End PCR Primer 1 (100% over 42bp)
AGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	17029	0.10145316511653055	Illumina Single End PCR Primer 1 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	39425	42.865036	1
CACTCTT	11930	42.639046	2
TGACTGG	39270	42.505806	2
GTGACCG	2205	42.27251	1
CCGTATC	9565	42.269825	43
CAGATAG	78585	42.263844	6
GTATCAT	9335	42.130287	45
AGATAGA	219395	42.003555	7
CCAGATA	22920	41.997177	5
GCAGATA	24840	41.840588	5
AGCACAC	250815	41.707047	17
GGCCTAG	2235	41.692913	2
CCTAGAT	13010	41.67351	4
GAGCACA	338880	41.667374	16
AGAGCAC	340820	41.65481	15
GCACACG	238345	41.647186	18
CTAGATA	17790	41.646576	5
ACACGTC	103390	41.59045	20
TGACCGG	2230	41.58453	2
CACACGT	168010	41.521847	19

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content