FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t2rr1r201.352000000589a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t2rr1r201.352000000589a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10092773
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4202544.163910156306894Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT245700.24344151998662808Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG169070.16751590469735125Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT127870.12669461603862486Illumina Single End PCR Primer 1 (100% over 39bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT119260.11816375935533277Illumina Single End PCR Primer 1 (100% over 47bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT113520.11247652156647138Illumina Single End PCR Primer 1 (97% over 49bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT110780.10976170770907064Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG504250.041.57124318
GAGCACA677500.041.32174316
AGCACAC530900.041.23894517
CACACGT359600.041.10005619
AGAGCAC685250.041.08711215
ACACGTC226650.040.5309220
CACACGA153300.040.09602719
ACACGAG152050.039.7449920
AAGAGCA855050.039.53419514
ACACGTA138250.039.13927520
CACGTAG136800.039.0936221
CACGTCT116400.039.0832821
GTCGCCG609350.038.81706245
AGATAGA339150.038.5692947
ACGTCAG112650.038.48693522
CACGAGA157700.038.33529321
CAGATAG125200.037.4320346
ACGTCTG87050.037.3733822
GGTCGCC635000.037.1605244
CACGTCA118900.037.0693921
Produced by FastQC (version 0.11.2)