FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t2r1r201.35200000058912.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t2r1r201.35200000058912.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12786322
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1849061.4461234434734243Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG184450.040.11830518
AGCACAC197200.038.7566317
GAGCACA248200.038.6430116
CACACGT145550.038.59790819
AGAGCAC253550.038.1114315
GTCGCCG309900.037.06978245
ACACGTC103150.036.9270920
GGTCGCC322950.035.46733544
CACGTCT62350.035.03806721
GTGGTCG328800.034.13834442
AGATAGA178600.033.6094747
ACACGAG48700.033.54015420
TGGTCGC339350.033.16321643
AAGAGCA358150.033.15506714
CACGTAG51050.033.05391321
CTCGGTG337050.032.9689838
GGTGGTC343150.032.70417841
CGGTGGT342950.032.63796240
GATAGAT182400.032.3412448
TCGGTGG343450.032.3348439
Produced by FastQC (version 0.11.2)