FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t1rr1r201p.352000000589f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t1rr1r201p.352000000589f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518060
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3654044.289756118177144Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT337670.3964165549432617Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT204990.24065338821280904Illumina Single End PCR Primer 1 (100% over 47bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT147500.17316149451870494Illumina Single End PCR Primer 1 (97% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG133000.15613883912534074Illumina Single End PCR Primer 1 (98% over 51bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT111210.1305578969859334Illumina Single End PCR Primer 1 (100% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG106610.12515760630941786Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG106540.1250754279730361Illumina Single End PCR Primer 1 (100% over 41bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC86910.10203027449912304Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG210950.040.5505118
CACACGT159600.040.1204519
AGCACAC222700.040.04766517
GAGCACA289100.039.6515216
AGAGCAC292500.039.45960215
ACACGTC107700.039.3576320
AGATAGA257950.038.3248677
CACACGA56100.037.4181719
CACGTCT61650.037.07869321
AAGAGCA386400.037.07158314
CAGATAG94900.036.8896566
ACACGTA56450.036.78760520
GTCGCCG616900.036.62450445
CACGTAG55750.036.60379821
CGTCTGA46700.036.4697323
ACACGAG56750.036.15703220
GATAGAT274850.036.0086368
ACGTCTG50150.035.39727822
ACGTCAG51500.035.3868222
GGTCGCC643750.035.01655644
Produced by FastQC (version 0.11.2)