FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t1rr1r201.3520000005898c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t1rr1r201.3520000005898c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11362488
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6462555.68761876800222Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT535980.47171006913274627Illumina Single End PCR Primer 1 (100% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT436310.3839916046556001Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG304450.26794307725561517Illumina Single End PCR Primer 1 (100% over 41bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT281810.24801786369323336Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT265470.23363721044193844Illumina Single End PCR Primer 1 (100% over 39bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG254600.22407064368296803Illumina Single End PCR Primer 1 (98% over 51bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT249710.21976700877483876Illumina Single End PCR Primer 1 (97% over 49bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG180560.15890885869362414Illumina Single End PCR Primer 1 (98% over 51bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC171780.15118167781563335Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC156400.13764590994507542Illumina Single End PCR Primer 1 (100% over 40bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC146960.12933787036782787Illumina Single End PCR Primer 1 (100% over 50bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG132860.11692861633825267Illumina Single End PCR Primer 1 (98% over 51bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG132110.11626854963455185Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG129540.11400672106320377Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC302350.041.21022820
GCACACG636500.041.15910318
CACACGT472600.041.11317419
CAGATAG290400.041.0388766
AGATAGA899450.040.9806187
AGCACAC672500.040.7389817
GAGCACA895050.040.4279616
AGAGCAC899550.040.348115
CACGTCT153800.039.90709321
TAGATAG265850.039.530776
ACACGTA171500.039.3698720
CACGTAG170150.039.21943321
ACGTCAG151550.039.20798522
GATAGAT943550.039.0287978
CACGTCA153900.038.94553821
CACACGA172050.038.7863219
AAGAGCA1167150.038.7513414
GATCGAG142750.038.692778
ACGTCTG115350.038.5807622
ACACGAG170900.038.33640720
Produced by FastQC (version 0.11.2)