FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t1r1r201.352000000588f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t1r1r201.352000000588f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8572976
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1976102.305033864552986Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT125580.14648355483556702Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG247550.040.84430318
GAGCACA336650.040.393116
AGAGCAC338450.040.3708215
CACACGT184300.040.10249319
AGCACAC264550.039.96308517
ACACGTC123450.038.49144420
ACACGAG70000.037.86244220
AAGAGCA435950.037.7045914
GTCGCCG296900.037.68531845
CACGTAG66550.037.25588621
CACACGA72800.036.9315819
ACACGTA68850.036.6648820
AGATAGA175550.036.602967
GGTCGCC307550.036.27791244
GTGGTCG308750.035.8672942
TGGTCGC312800.035.37412343
CACGTCT69050.035.35309221
ACGTCAG60000.035.21078522
CACGAGA75450.035.12751421
GATAGAT181900.035.077398
Produced by FastQC (version 0.11.2)