FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t0rr1r201p.352000000589e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t0rr1r201p.352000000589e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14080
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG760.5397727272727273Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGACT206.797805E-445.0000042
CACACGT1300.045.00000419
GAAATTG206.797805E-445.00000424
CGGGGGC206.797805E-445.00000435
TTCCGAC206.797805E-445.00000428
AGGCGAG206.797805E-445.00000416
CTGAACA302.0159514E-645.00000426
TCGCCGT206.797805E-445.00000445
AACTAGA406.028131E-945.00000429
GCGCAGA206.797805E-445.00000412
TGGCGGA206.797805E-445.0000048
CAGGCGA206.797805E-445.00000415
AGCGCAG302.0159514E-645.00000411
GGGAAAT206.797805E-445.00000422
TGAACTA406.028131E-945.00000427
TTGTGTA302.0159514E-645.00000428
GCACACG1450.045.00000418
GTCAGGC206.797805E-445.00000413
AATTGTG302.0159514E-645.00000426
CGCCGAG302.0159514E-645.00000413