FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t0rr1r201.35200000058956.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t0rr1r201.35200000058956.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10513108
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9378698.920948971512516Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT740240.7041114768344433Illumina Single End PCR Primer 1 (100% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT463020.44042161461672424Illumina Single End PCR Primer 1 (100% over 51bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT416610.39627672425699423Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG398040.37861306095209907Illumina Single End PCR Primer 1 (100% over 41bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT347290.3303399908000565Illumina Single End PCR Primer 1 (97% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC326610.31066930920903696Illumina Single End PCR Primer 1 (100% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG308050.29301515783914706Illumina Single End PCR Primer 1 (98% over 51bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC275720.2622630719669198Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT259560.2468917849983088Illumina Single End PCR Primer 1 (100% over 39bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG246400.23437407853129635Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG225430.2144275508251223Illumina Single End PCR Primer 1 (98% over 51bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG206530.1964499936650513Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG202440.19255961224787188Illumina Single End PCR Primer 1 (98% over 51bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC183870.17489594894297672Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC170270.16195971733572984Illumina Single End PCR Primer 1 (100% over 40bp)
AGGTTGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG156350.14871910380831244Illumina Single End PCR Primer 1 (100% over 46bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG127800.12156252936809933Illumina Single End PCR Primer 1 (98% over 51bp)
AGGGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG111570.10612465885445103Illumina Single End PCR Primer 1 (100% over 47bp)
AGAGTGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG110970.10555394275413132Illumina Single End PCR Primer 1 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG365000.041.3917476
GCACACG714900.041.31282818
AGATAGA1094300.041.2928127
ACACGTC321300.041.20979720
AGCACAC750550.041.203117
CACACGT502650.041.1263419
AGAGCAC1027750.040.8294215
GAGCACA1022650.040.7828116
GTGACTG142000.040.7038041
TAGATAG319000.040.455646
CACGTCT154450.040.32208321
TGACTGG140350.040.240152
CACACGA219200.040.10214619
ACACGAG218950.039.6855220
CACGTCA166550.039.67564821
AAGAGCA1372700.039.53228814
GATCGAG167350.039.4583448
ACACGTA182800.039.33646420
ACGTCAG166950.039.28410722
GATAGAT1151900.039.198328
Produced by FastQC (version 0.11.2)