FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n02_t0r1r201.352000000588ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n02_t0r1r201.352000000588ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10187786
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG667180.6548822285823436Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG67300.035.70342618
GTCGCCG120300.034.5616445
CACACGT58000.033.3986519
GAGCACA98200.032.9458816
GGTCGCC126400.032.80471444
AGCACAC77600.032.50106417
AGAGCAC102000.032.04918715
ACACGTC43950.031.27778820
GTGGTCG133800.030.63698242
AGATAGA104250.030.1929827
CTCGGTG134100.030.08189838
CACGTCT33050.029.47610721
GATAGAT107000.029.1638138
TCGGTGG142350.028.4333139
ATAGATC109300.028.3031089
GGTGGTC145700.028.19648441
TCTCGGT144000.027.98265537
TGGTCGC146700.027.95840643
ACGTCTG28000.027.72137522
CGGTGGT148950.027.4755240
Produced by FastQC (version 0.11.2)